CRISPR editing of human cells- hands-on class

Description

This community project will use CRISPR (-associated endonucleases) to edit the DNA of human cell lines. We will meet every week on Thursday at 7pm, but we will only take new members during posted meetup classes (typically the first Thursday of the month). During the first meeting, we will orient our new members to the project. In subsequent weeks, we will make CRISPR (-associated proteins) guide RNAs, clone them into a mammalian expression vector, and then transfect Cas9 protein with the guide RNA into human cells. Using this we should be able to knock out our targeted gene in an appropriate cell line. The cell lines we generate will be open source and available for any community lab or project.

You do not need any prior experience or knowledge to join. This community project will primarily take place in the laboratory. You must wear close-toed shoes to go into the lab. Any minors must be accompanied by their parent or guardian. Please complete the sign-in and liability waiver on the tablet when you come in.

Please make sure to leave your contact info with the organizers so that we can add you to the mailing list for meetings in future weeks (Thursdays at 7pm).

You can park out front anywhere, please try to stay on the east side of the front lot. Come on in the front door, and just keep going through that first lobby. If you're early, come in and hang out, feel free to sit on the sofa or at the tables. There's bathrooms to the left behind the partition, and there's also a kitchen on the left where you can get water and we have a few snacks available (we ask a small donation to pay Joe back for buying snacks for everyone!).

BioCurious is a community biolab where students, start-ups, small businesses, independent researchers, and curious minds converge to create. We run at BSL-1 containment under the supervision of our Safety Committee.